Detection of Disulfide-Linked Peptides by HPLC
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Classical techniques for determining disulfide bond patterns usually require the fragmentation of proteins into peptides under low-pH conditions to prevent disulphide exchange. Pepsin or cyanogen bromide are particularly useful (see Chapter 63). Diagonal techniques to identify disulphide-linked peptides were developed by Brown and Hartley (see Chapter 81). A modern micromethod employing reverse-phase HPLC is described here.
KeywordsCyanogen Bromide Iodoacetic Acid Peptide Bond Cleavage Reverse Phase HPLC Column Reductive Alkylation