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Amino Acid Analysis Using Precolumn Derivatization with Phenylisothiocyanate

  • G. Brent Irvine
Protocol
Part of the Springer Protocols Handbooks book series (SPH)

Abstract

The method described in this chapter is based on derivatization of amino acids, produced by hydrolysis of peptides or proteins, with phenylisothiocyanate. This forms phenylthiocarbamyl amino acids, which are then separated by reversed-phase high-performance liquid chromatography and quantified from their UV absorbance at 254 nm.

Keywords

Cold Trap Sodium Dihydrogen Phosphate Methanesulfonic Acid Amino Acid Standard Dansyl Chloride 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Humana Press Inc., Totowa, NJ 1996

Authors and Affiliations

  • G. Brent Irvine
    • 1
  1. 1.School of Biology and BiochemistryQueen’s University of BelfastIreland

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