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Protein Quantitation Using Flow Cytometry

  • F. Andrew Ray
  • Thomas D. Friedrich
  • Judith Laffin
  • John M. Lehman
Protocol
  • 99 Downloads
Part of the Springer Protocols Handbooks book series (SPH)

Abstract

It is often desirable to know how much of a particular protein is produced by a particular cell. For example, is this protein expressed at low levels throughout a population of cells, or is this protein expressed at very high levels in some cells and not in others? Does the expression of the protein change in a particular cell-cycle compartment? Technical protocols that require cell lysis, such as immunoblotting (1), allow the mean expression level of a given protein to be compared between populations of cells. It is even possible to use the immunoblotting protocol to determine the amount of a given protein expressed by a population of cells, if the protein has been purified for use as a standard. However, cell lysis obliterates the potential for single-cell quantitation of proteins.

Keywords

Propidium Iodide Repeat Centrifugation Flow Cytometer System Target Protein Molecule Minimize Cell Loss 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Humana Press Inc., Totowa, NJ 1996

Authors and Affiliations

  • F. Andrew Ray
    • 1
  • Thomas D. Friedrich
    • 1
  • Judith Laffin
    • 1
  • John M. Lehman
    • 1
  1. 1.Department of Microbiology, Immunology, and Molecular GeneticsThe Albany Medical CollegeAlbany

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