Side-Chain Selective Chemical Modifications of Proteins

  • Dan S. Tawfik
Part of the Springer Protocols Handbooks book series (SPH)


Chemical modifications of proteins may be performed simply and rapidly, and can readily provide preliminary data regarding the role of particular amino acids in a given protein. Even in the era of molecular biology, when site-directed mutagenesis has become so popular, selective chemical modifications are often useful. Many reviews and books cover these aspects of protein chemistry; only a few are cited here (1, 2, 3, 4). In particular, the book by Means and Feeney (1), although more than 20 years old, is an excellent introduction and a practical guide to this field.


Amino Acid Side Chain Modification Reaction Diethyl Pyrocarbonate Protein Chemistry Modify Side 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.


  1. 1.
    Means, G. E. and Feeney, R. E. (1971) Chemical Modifications of Proteins. Holden-Day, San Francisco, CA.Google Scholar
  2. 2.
    Hirs, C. H. N. and Timasheff, S. N. (eds.) (1972) Enzyme Structure B. Methods Enzymology 25.Google Scholar
  3. 3.
    Lundblad, R. L. and Noyes, C. M. (1984) Chemical Reagents for Protein Modifications, vols. 1 and 2. CRC, Boca Raton, FL.Google Scholar
  4. 4.
    Feeney, R. E. (1987) Chemical modification of proteins: comments and perspectives. Int. J. Pept. Protein Res. 27, 145–161.Google Scholar

Copyright information

© Humana Press Inc., Totowa, NJ 1996

Authors and Affiliations

  • Dan S. Tawfik
    • 1
  1. 1.Department of Chemical ImmunologyWeizmann Institute of ScienceRehovotIsrael

Personalised recommendations