Side-Chain Selective Chemical Modifications of Proteins
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Chemical modifications of proteins may be performed simply and rapidly, and can readily provide preliminary data regarding the role of particular amino acids in a given protein. Even in the era of molecular biology, when site-directed mutagenesis has become so popular, selective chemical modifications are often useful. Many reviews and books cover these aspects of protein chemistry; only a few are cited here (1, 2, 3, 4). In particular, the book by Means and Feeney (1), although more than 20 years old, is an excellent introduction and a practical guide to this field.
KeywordsAmino Acid Side Chain Modification Reaction Diethyl Pyrocarbonate Protein Chemistry Modify Side
- 1.Means, G. E. and Feeney, R. E. (1971) Chemical Modifications of Proteins. Holden-Day, San Francisco, CA.Google Scholar
- 2.Hirs, C. H. N. and Timasheff, S. N. (eds.) (1972) Enzyme Structure B. Methods Enzymology 25.Google Scholar
- 3.Lundblad, R. L. and Noyes, C. M. (1984) Chemical Reagents for Protein Modifications, vols. 1 and 2. CRC, Boca Raton, FL.Google Scholar
- 4.Feeney, R. E. (1987) Chemical modification of proteins: comments and perspectives. Int. J. Pept. Protein Res. 27, 145–161.Google Scholar