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Detection of Proteins in Polyacrylamide Gels by Silver Staining

  • Michael J. Dunn
Protocol
Part of the Springer Protocols Handbooks book series (SPH)

Abstract

The versatility and resolving capacity of polyacrylamide gel electrophoresis (PAGE) has resulted in this group of methods becoming the most popular for the analysis of patterns of gene expression in a wide variety of complex systems. These techniques are often used to characterize protein purity and to monitor the various steps in a protein purification process. Moreover, with the advent of sensitive methods for chemical characterization, gel electrophoresis has become one of the most important methods of protein purification.

Keywords

Sodium Dodecyl Sulfate Silver Staining Silver Nitrate Background Staining Dilute Hydrochloric Acid 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Humana Press Inc., Totowa, NJ 1996

Authors and Affiliations

  • Michael J. Dunn
    • 1
  1. 1.Department of Cardiothoracic SurgeryNational Heart and Lung Institute, Heart Science Centre, Harefield HospitalMiddlesexUK

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