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Drying Polyacrylamide Gels

  • Bryan John Smith
Protocol
Part of the Springer Protocols Handbooks book series (SPH)

Abstract

Gels can be fragile and difficult to handle and store without suffering damage. This can be overcome by drying, whereby they become bonded to a stabilizing medium. Furthermore, it may be necessary to dry a gel in order to obtain the most efficient detection of radioactive samples on it by autoradiography, and fluorography. In this chapter, two methods are described—the first being drying onto absorbent paper (this being suitable for storage, reflection densitometry, and autoradiography), and the second being drying between cellulose sheets (this being suitable for storage, transmission densitometry, and demonstration by overhead projection). Both methods are suitable for slab gels—tube-shaped gels are not suited to drying down.

Keywords

Silicon Rubber Polyethylene Sheet Rubber Sheet Absorbent Paper Overhead Projection 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

References

  1. 1.
    Joshi, S. and Haenni, A. L. (1980) Fluorographic detection of nucleic acids labeled with weak β-emitters in gels containing high acrylamide concentrations. FEES Lett. 118, 43–46.CrossRefGoogle Scholar
  2. 2.
    Bio-Rad Inc. (1981) Model 1125B high capacity gel slab dryer for protein gels and DNA sequencing. Bio-Rad Bulletin 1079, Bio-Rad Inc., Hercules, CA.Google Scholar

Copyright information

© Humana Press Inc., Totowa, NJ 1996

Authors and Affiliations

  • Bryan John Smith
    • 1
  1. 1.Celltech Therapeutics Ltd.SloughUK

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