Advertisement

Protein Staining with Calconcarboxylic Acid in Polyacrylamide Gels

  • Jung-Kap Choi
  • Hee-Youn Hong
  • Gyurng-Soo Yoo
Protocol
Part of the Springer Protocols Handbooks book series (SPH)

Abstract

Sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) has become a highly reliable separation technique for protein characterization. Broad application of electrophoresis techniques has required the development of detection methods that can be used to visualize the proteins separated on polyacrylamide gels. A few of these methods are Coomassie brilliant blue (CBB) staining (1 and see Chapter 11), silver staining (2 and see Chapter 35), fluorescent staining (3, 4, 5), specific enzyme visualization (6), and radioactive detection (7 and see Chapter 25 and 36).

Keywords

Coomassie Brilliant Blue Methanol Content Dansyl Chloride Coomassie Brilliant Blue Staining Radioactive Detection 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

References

  1. 1.
    Fazekas de St. Groth, S., Webster, R. G., and Datyner, A. (1963) Two new staining procedures for quantitative estimation of proteins on electrophoretic strips. Biochim. Biophys. Acta 71, 377–391.PubMedCrossRefGoogle Scholar
  2. 2.
    Merril, C. R., Goldman, D., Sedman, S., and Ebert, M. (1980) Ultrasensitive stain for proteins in polyacrylamide gels shows regional variation in cerebrospinal fluid proteins. Science 211, 1437,1438.CrossRefGoogle Scholar
  3. 3.
    Schetters, H. and McLeod, B. (1979) Simultaneous isolation of major viral proteins in one step. Anal. Biochem. 98, 329–334.PubMedCrossRefGoogle Scholar
  4. 4.
    Jakowski, G. and Liew, C. C. (1980) Fluorescamine staining of nonhistone chromatin proteins as revealed by two-dimensional polyacrylamide gel electrophoresis. Anal. Biochem. 102, 321–325.CrossRefGoogle Scholar
  5. 5.
    Weiderkamm, E., Wallach, D. F. H., and Fluckiger, R. (1973) A new sensitive, rapid fluorescence technique for the determination of proteins in gel electrophoresis and in solution. Anal. Biochem. 54, 102–114.CrossRefGoogle Scholar
  6. 6.
    Gabriel, O. (1971) Locating enzymes on gels in Methods in Enzymology, vol. 22 (Colowick, S. P. and Kaplan, N. O., eds.), Academic, New York, pp. 363–367.Google Scholar
  7. 7.
    O’Farrell, P. H. (1975) High resolution two-dimensional electrophoresis of proteins. J. Biol. Chem. 250, 4007–4021.PubMedGoogle Scholar
  8. 8.
    Diezel, W., Kopperschlager, G., and Hofmann, E. (1972) An improved procedure for protein staining in polyacrylamide gels with a new type of Coomassie Brilliant Blue. Anal. Biochem. 48, 617–620.PubMedCrossRefGoogle Scholar
  9. 9.
    Zehr, B. D., Savin, T. J., and Hall, R. E. (1989) A one-step low background Coomassie staining procedure for polyacrylamide gels. Anal. Biochem. 182, 157–159.PubMedCrossRefGoogle Scholar
  10. 10.
    Hames, B. D. and Rickwood, D. (1990) Analysis of gels following electrophoresis, in Gel Electrophoresis of Proteins: A Practical Approach, IRL, Oxford, UK, pp. 52–81.Google Scholar
  11. 11.
    Switzer, R. C., Merril, C. R., and Shifrin, S. (1979) A highly sensitive silver stain for detecting proteins and peptides in polyacrylamide gels. Anal. Biochem. 98, 231–237.PubMedCrossRefGoogle Scholar
  12. 12.
    Ohsawa, K. and Ebata, N. (1983) Silver stain for detecting 10-femtogram quantities of protein after polyacrylamide gel electrophoresis. Anal. Biochem. 135, 409–415.PubMedCrossRefGoogle Scholar
  13. 13.
    Hong, H. Y., Yoo, G. S., and Choi, J. K. (1993) Detection of proteins on polyacrylamide gels using calconcarboxylic acid. Anal. Biochem. 214, 96–99.PubMedCrossRefGoogle Scholar
  14. 14.
    Borejdo, J. and Flynn, C. (1984) Electrophoresis in the presence of Coomassie Brilliant Blue R-250 stains polyacrylamide gels during protein fractionation. Anal. Biochem. 140, 84–86.PubMedCrossRefGoogle Scholar

Copyright information

© Humana Press Inc., Totowa, NJ 1996

Authors and Affiliations

  • Jung-Kap Choi
    • 1
  • Hee-Youn Hong
    • 1
  • Gyurng-Soo Yoo
    • 1
  1. 1.College of PharmacyChonnam National UniversityKwangjuKorea

Personalised recommendations