Purification of IgG Using Affinity Chromatography on Antigen-Ligand Columns
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The antigen recognized by the IgG (for isolation of the antigen-specific fraction of the serum/ascitic fluid, and so forth).
IgG prepared from an anti-immunoglobulin serum, e.g., rabbit antihuman IgG serum or murine antihuman IgG MAb for the purification of human IgG (see Note 1).
IgG-binding proteins derived from bacteria, e.g., protein A (from Staphylococcus aureus Cowan 1 strain) or proteins G or C (from Streptococcus and see Chapter 130).
KeywordsSodium Citrate Buffer Fume Hood Guanidine Hydrochloride Trisodium Citrate Cyanogen Bromide
- 1.Hermanson, G. T., Mallia, A. K., and Smith, P. K. (1992) Immobilized Affinity Ligand Techniques. Academic, San Diego, CA.Google Scholar