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Purification of IgG by Precipitation with Polyethylene Glycol (PEG)

  • Mark Page
  • Robin Thorpe
Protocol
Part of the Springer Protocols Handbooks book series (SPH)

Abstract

PEG precipitation works well for IgM, but is less efficient for IgG; salt precipitation methods are usually recommended for IgG. PEG precipitation may be preferred in multistep purifications that use ion-exchange columns, because the ionic strength of the Ig is not altered. Furthermore, it is a very mild procedure that usually results in little denaturation of antibody. This procedure is applicable to both polyclonal antisera and most MAb containing fluids.

Keywords

Ionic Strength Ammonium Sulfate Polyethylene Glycol Precipitation Method Sulfate Precipitation 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Copyright information

© Humana Press Inc., Totowa, NJ 1996

Authors and Affiliations

  • Mark Page
    • 1
  • Robin Thorpe
    • 2
  1. 1.MEDEVA, Vaccine Research Unit, Department of BiochemistryImperial College of Science, Technology, and MedicineLondonUK
  2. 2.National Institute for Biological Standards and ControlPotters BarUK

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