Purification of IgG Using Caprylic Acid
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Caprylic (octanoic) acid can be used to purify mammalian IgG from serum, plasma, ascites fluid, and hybridoma culture supernatant by precipitation of non-IgG protein (1) (see Note 1). Other methods have been described where caprylic acid has been used to precipitate immunoglobulin depending on the concentration used. The concentration of caprylic acid required to purify IgG varies according to species (see Section 3., step 2). For MAb, it is usually necessary to determine experimentally the quantity required to produce the desired purity/yield. Generally, the product is of low to intermediate purity but this will depend on the starting material. Caprylic acid purified IgG preparations can be used for most immunochemical procedures, such as coating plates for antigen capture assays and preparation of immunoaffinity columns, but would not be suitable for conjugation with radioisotopes, enzymes, and biotin where contaminating proteins will reduce the specific activity.