Elution of SDS-PAGE Separated Proteins from Immobilon Membranes for Use as Antigens
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The great analytical power of sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) makes it one of the most effective tools of protein chemistry and molecular biology (see Chapter 11). In the past, there have been many attempts to convert the technique from analytical to preparative scale because, by SDS-PAGE, one can resolve more than one hundred protein species in 5–6 h. The number of papers that describe preparative elution from polyacrylamide gels is immense (e.g., see refs. 1, 2, 3, 4, 5). In spite of the numerous variations in the procedure of elution, none of the available methods is entirely satisfactory. Some of the methods are very laborious, and others lead to loss of resolution or poor recovery.
KeywordsPreparative Scale Precise Excision Immobilon Membrane Large Complex Enzyme Nitrocellulose Replica
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