Determination of Monosaccharide Linkage and Substitution Patterns by GC-MS Methyiation Analysis
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The GC or GC-MS method discussed in Chapter 102 can distinguish substituted monosaccharides, but to characterize the position of acyl groups together with the linkages between the monosaccharides, a strategy has been developed to “capture” the substitution pattern by methyiation of all free hydroxyl groups. The constituent monosaccharides are then analyzed after hydrolysis, reduction, and acetylation as partially methylated alditol acetates in a procedure known as methyiation analysis (1, 2, 3).
KeywordsMethyl Iodide Substitution Pattern Constituent Monosaccharide Acetamido Group Hexose Residue