Protein Folding, Misfolding, and Disease pp 45-57

Part of the Methods in Molecular Biology book series (MIMB, volume 752) | Cite as

Refolding Your Protein with a Little Help from REFOLD

  • Jennifer Phan
  • Nasrin Yamout
  • Jason Schmidberger
  • Stephen P. Bottomley
  • Ashley M. Buckle
Protocol

Abstract

The expression and harvesting of proteins from insoluble inclusion bodies by solubilization and refolding is a technique commonly used in the production of recombinant proteins. Despite the importance of refolding, publications in the literature are essentially ad hoc reports consisting of a dazzling array of experimental protocols and a diverse collection of buffer cocktails. For the protein scientists, using this information to refold their protein of interest presents enormous challenges. Here, we describe some of the practical considerations in refolding and present several standard protocols. Further, we describe how refolding procedures can be designed and modified using the information in the REFOLD database (http://refold.med.monash.edu.au), a freely available, open repository for protocols describing the refolding and purification of recombinant proteins.

Key words

Protein expression Refolding Renaturation Inclusion body Aggregate Refold Misfolding Solubilization 

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Copyright information

© Springer Science+Business Media, LLC 2011

Authors and Affiliations

  • Jennifer Phan
  • Nasrin Yamout
  • Jason Schmidberger
  • Stephen P. Bottomley
  • Ashley M. Buckle
    • 1
  1. 1.Department of Biochemistry and Molecular BiologyMonash UniversityClaytonAustralia

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