Study Origin of Germ Cells and Formation of New Primary Follicles in Adult Human and Rat Ovaries

  • Antonin Bukovsky
  • Satish K. Gupta
  • Irma Virant-Klun
  • Nirmala B. Upadhyaya
  • Pleas Copas
  • Stuart E. Van Meter
  • Marta Svetlikova
  • Maria E. Ayala
  • Roberto Dominguez
Part of the Methods in Molecular Biology™ book series (MIMB, volume 450)

Summary

The central thesis regarding the human ovaries is that, although primordial germ cells in embryonal ovaries are of extraovarian origin, those generated during the fetal period and in postnatal life are derived from the ovarian surface epithelium (OSE) bipotent cells. With the assistance of immune system-related cells, secondary germ cells and primitive granulosa cells originate from OSE stem cells in the fetal and adult human gonads. Fetal primary follicles are formed during the second trimester of intrauterine life, prior to the end of immune adaptation, possibly to be recognized as self-structures and renewed later. With the onset of menarche, a periodical oocyte and follicular renewal emerges to replace aging primary follicles and ensure that fresh eggs for healthy babies are always available during the prime reproductive period. The periodical follicular renewal ceases between 35 and 40 yr of age, and the remaining primary follicles are utilized during the premenopausal period until exhausted. However, the persisting oocytes accumulate genetic alterations and may become unsuitable for ovulation and fertilization. The human OSE stem cells preserve the character of embryonic stem cells, and they may produce distinct cell types, including new eggs in vitro, particularly when derived from patients with premature ovarian failure or aging and postmenopausal ovaries. Our observations also indicate that there are substantial differences in follicular renewal between adult human and rat ovaries. As part of this chapter, we present in detail protocols utilized to analyze oogenesis in humans and to study interspecies differences when compared to the ovaries of rat females.

Keywords

Adulthood follicular renewal humans mammals oogenesis 

Notes

Acknowledgments

Acknowledgments We thank Drs. Bonnie S. Dunbar and Sarvamangala V. Prasad of the Department of Molecular and Cellular Biology, Baylor College of Medicine, Houston, Texas, for kindly supplying additional ZP1,2,3 antibodies and Dr. A. Neil Barclay of the Sir William Dunn School of Pathology, University of Oxford, Oxford, United Kingdom, who kindly provided mouse antirat Ia and LCA monoclonal antibodies. Peroxidase conjugate of swine antimouse IgG was kindly provided by Dr. Jana Peknicova of the Department of Biology and Biochemistry of Fertilization, Institute of Molecular Genetics, Academy of Sciences of the Czech Republic, Prague, and HLA-DR was kindly donated by Drs. Ivan Hilgert and Vaclav Horejsi of the Institute of Molecular Genetics, Academy of Sciences and Faculty of Sciences, Charles University, Prague, Czech Republic. Clone F15-42-01 to human Thy-1dp was kindly donated by Dr. Rosemarie Dalchau, Institute of Child Health, University of London. We also thank Drs. Helena Meden-Vrtovec, Tomaž Tomaževič, Jasna Šinkovec, and Andrej Vogler of the Department of Obstetrics and Gynecology, University of Ljubljana Medical Center, for stimulating suggestions and support, as well as excellent laparoscopic collection of OSE cells and ovarian biopsies by Dr. Andrej Vogler. This work was supported by the Physicians' Medical Education and Research Foundation award to A.B.

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Copyright information

© Humana Press, a part of Springer Science + Business Media, LLC 2008

Authors and Affiliations

  • Antonin Bukovsky
    • 1
  • Satish K. Gupta
    • 2
  • Irma Virant-Klun
    • 3
  • Nirmala B. Upadhyaya
    • 4
  • Pleas Copas
    • 4
  • Stuart E. Van Meter
    • 5
  • Marta Svetlikova
    • 1
  • Maria E. Ayala
    • 6
  • Roberto Dominguez
    • 6
  1. 1.Laboratory of Development, Differentiation and Cancer, Department of Obstetrics and GynecologyThe University of Tennessee Graduate School of MedicineKnoxvilleUSA
  2. 2.Gamete Antigen LaboratoryNational Institute of ImmunologyNew DelhiIndia
  3. 3.Department of Obstetrics and GynecologyUniversity Medical CentreLjubljanaSlovenia
  4. 4.Department of Obstetrics and GynecologyThe University of Tennessee Graduate School of MedicineKnoxvilleUSA
  5. 5.Department of PathologyThe University of Tennessee Graduate School of MedicineKnoxvilleUSA
  6. 6.Unidad de Investigacion en Biologia de la Reproduccion, FES ZaragozaUNAMMexico

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