Germline Stem Cells pp 181-192

Part of the Methods in Molecular Biology™ book series (MIMB, volume 450)

Deriving Mouse Spermatogonial Stem Cell Lines

  • Ilaria Falciatori
  • Kate Lillard-Wetherell
  • Zhuoru Wu
  • F. Kent Hamra
  • David L. Garbers

Summary

In adult males, spermatogonial stem cells function to replenish developing gametes that are continuously released from the testes as mature spermatozoa. Because of their potential importance to research, medicine, industry, and conservation, numerous attempts have been made in the past to cultivate sperma-togonial stem cells in vitro. However, only recently have culture methods been established that effectively promote the proliferation of mammalian spermatogonial stem cells in vitro. We describe a simple and reproducible protocol for the derivation and maintenance of mouse spermatogonial stem cell lines that proliferate for long periods of time in culture.

Keywords

Germ cells germline stem cells mouse spermatogonia spermatogonial stem cells spermatogonial stem cell culture SSC testis 

Copyright information

© Humana Press, a part of Springer Science + Business Media, LLC 2008

Authors and Affiliations

  • Ilaria Falciatori
    • 1
  • Kate Lillard-Wetherell
    • 2
  • Zhuoru Wu
    • 3
  • F. Kent Hamra
    • 4
  • David L. Garbers
    • 5
  1. 1.Department of PharmacologyHoward Hughes Medical Institute, University of Texas Southwestern Medical CenterDallasUSA
  2. 2.Department of PharmacologyHoward Hughes Medical Institute, University of Texas Southwestern Medical CenterDallasUSA
  3. 3.Department of PharmacologyUniversity of Texas Southwestern Medical CenterDallasUSA
  4. 4.Department of Pharmacology and Cecil H. and Ida Green Center for Reproductive Biology SciencesUniversity of Texas Southwestern Medical CenterDallasUSA
  5. 5.Department of Pharmacology and Cecil H. and Ida Green Center for Reproductive Biology SciencesHoward Hughes Medical Institute, University of Texas Southwestern Medical CenterDallasUSA

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