Microarray Analysis of the Physical Genome pp 89-98

Part of the Methods in Molecular Biology™ book series (MIMB, volume 556) | Cite as

A Whole-Genome Amplification Protocol for a Wide Variety of DNAs, Including Those from Formalin-Fixed and Paraffin-Embedded Tissue

  • Pamela L. Paris
Protocol

Abstract

High-resolution genomic arrays and next-generation sequencers are some of the genome-based technologies poised to make significant contributions in the near future to basic and clinical science. The success of these technologies, and most certainly their translation into the clinic, will require that they produce high quality, reproducible data from small archived tumor specimens, including biopsies. DNA from patient samples, especially archival tissue, can be a limiting factor and lead to the need for amplification of the starting material. A variety of whole-genome amplification techniques are available, but choosing the most reliable, reproducible amplification technology that will be suitable for use across a wide spectrum of clinical specimens is essential. Sigma’s whole-genome amplification kit provides a robust, highly reliable, and versatile amplification system across a variety of DNA sources. This chapter will detail Sigma’s amplification protocol along with an optimized DNA extraction protocol for formalin-fixed and paraffin-embedded tissue.

Key words

Whole-genome amplification clinical specimens formalin-fixed paraffin-embedded DNA extraction array comparative genomic hybridization (aCGH) 

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Copyright information

© Humana Press, a part of Springer Science+Business Media, LLC 2009

Authors and Affiliations

  • Pamela L. Paris
    • 1
  1. 1.Department of UrologyUniversity of California at San FranciscoSan FranciscoUSA

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