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Four-Vessel Occlusion Model in Rats

  • Ping Deng
  • Zao C. Xu
Protocol
Part of the Springer Protocols Handbooks book series (SPH)

Four-vessel occlusion model in rat induces transient forebrain ischemia that resembles cardiac arrest in clinical situations. In this model, two vertebral arteries are coagulated and two common carotid arteries are revers-ibly occluded. This model produces reliable outcome with selective, delayed cell death. For example, CA1 pyramidal neurons in the hippocampus die 2–3 days after ischemia, whereas CA3 neurons survive the same insult. The complications of this model include mortality and seizure. The methods of quantitative analysis of ischemic outcome are introduced. The advantages and limitations of this model are discussed.

Keywords

Wistar rat Transient global ischemia Hippocampus Striatum Ischemic depolarization 

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Copyright information

© Humana Press, a part of Springer Science + Business Media, LLC 2009

Authors and Affiliations

  • Ping Deng
    • 1
  • Zao C. Xu
    1. 1.Department of Anatomy & Cell BiologyIndiana University School of MedicineIndianapolisUSA

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