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Blood Injection Cerebral Vasospasm Rabbit Model

  • Mario Zuccarello
Protocol
  • 2k Downloads
Part of the Springer Protocols Handbooks book series (SPH)

Cerebral vasospasm after subarachnoid hemorrhage (SAH) is one of the major causes of cerebral ischemia, and is the leading cause of death and disability following aneurysm rupture. The pathogenesis of cerebral vasospasm is poorly understood, and numerous hypotheses have been proposed to explain its underlying cause. The development of an animal model able to mimic the human condition is extremely useful to try to understand the pathogenesis of post-SAH cerebral vasospasm, ultimately leading to more efficacious treatment. We introduce step-by-step procedures to create a double-hemorrhage model of SAH in rabbits. We also performed angiographic studies of the vertebrobasilar vasculature and measured the diameter of the basilar artery to quantify the post-SAH vasospasm.

Keywords

Rabbit Subarachnoid hemorrhage Cerebral vasospasm 

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References

  1. 1.
    Zuccarello M, Lewis IA, Rapoport RM. ETAand ETBreceptors in subarachnoid hemorrhage-induced cerebral vasospasm.Eur J Pharmacol1994;259:R1–R2.PubMedCrossRefGoogle Scholar
  2. 2.
    Zuccarello M, Lewis IA, Upputuri S, Anderson DK. Effect of remacemide on subara-chnoid hemorrhage-induced vasospasm in rabbits.J Neurotrauma1994:11:691–698.PubMedCrossRefGoogle Scholar
  3. 3.
    Zuccarello M, Romano A, Passalacqua M, Rapoport RM. Endothelin-1 induced endothelin-1 release causes cerebral vasospasm.J Pharm Pharmacol1995;47:702.PubMedGoogle Scholar
  4. 4.
    Zuccarello M, Romano A, Passalacqua M, Rapoport RM. Decreased endothelium-dependent relaxation in subarachnoid hemorrhage-induced vasospasm: role of endothelin-1.Am J Physio, 1995;269:H1009–H1015.Google Scholar
  5. 5.
    Zuccarello M, Soattin GB, Lewis AI, Breu V, Hallak H, Rapoport RM: Prevention of subarachnoid hemorrhage-induced vasospasm by oral administration of endothelin receptor antagonists.J Neurosurg, 1996;84:503–507.PubMedGoogle Scholar
  6. 6.
    Zuccarello M, Boccaletti R, Rapoport RM: Role of extracellular Ca2+ in sub-arachnoid hemorrhage-induced vasospasm of the rabbit basilar artery.Stroke, 1996;27:1896–1902.PubMedGoogle Scholar
  7. 7.
    Zuccarello M, Boccaletti R, Rapoport RM: Does blockade of EndothelinB1 receptor activation increase EndothelinB2/EndothelinAreceptor -mediated constriction in the rabbit basilar artery.J Cardiovasc Pharmacol1999;3:679–684.CrossRefGoogle Scholar
  8. 8.
    Byung Ho Lee, Sherman JD, Zuccarello M, Rapoport RM: Vasospasm following subarachnoid hemorrhage: evidence against functional upregulation of PKC constrictor pathway.Neurol Res2003;25(3):268–270.CrossRefGoogle Scholar

Copyright information

© Humana Press, a part of Springer Science + Business Media, LLC 2009

Authors and Affiliations

  • Mario Zuccarello
    • 1
  1. 1.Department of Neurosurgery, The Neuroscience InstituteUniversity of Cincinnati School of Medicine, and Mayfi eld ClinicCincinnatiUSA

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