Blood Injection Subarachnoid Hemorrhage Mouse Model
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Cerebral vasospasm is a major complication of subarachnoid hemorrhage (SAH). This chapter describes a reproducible, low-mortality, and low-cost model of SAH-induced vasospasm in the mouse. Autologous blood is injected into the cisterna magna of adult mice, and the diameters of large cerebral arteries are measured postmortem. Acute (6–12 h) and persistent (1–3 days) phases of vascular narrowing are observed in cerebral arteries. Vascular narrowing is associated with thickening of the vascular wall and corrugation of the internal elastic lamina; these histological findings have been described by Lin and associates.1 A key advantage of this model is the use of a species for which a variety of resources are available, such as a range of genetically-modified animals. This mouse model could therefore assist in identifying and verifying mechanisms contributing to vascular pathology associated with SAH and facilitate the initial screening of candidate therapies for cerebral vasospasm.
KeywordsSubarachnoid hemorrhage Cerebral vasospasm Mouse Vasoconstriction Stroke
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