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Blood Injection Subarachnoid Hemorrhage Mouse Model

  • Tarkan Calisaneller
  • Chih-Lung Lin
  • Naoya Ukita
  • Ryo Yoshimura
  • Yi Wang
  • Mark Fitzgerald
  • Kevin S. Lee
Protocol
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Part of the Springer Protocols Handbooks book series (SPH)

Cerebral vasospasm is a major complication of subarachnoid hemorrhage (SAH). This chapter describes a reproducible, low-mortality, and low-cost model of SAH-induced vasospasm in the mouse. Autologous blood is injected into the cisterna magna of adult mice, and the diameters of large cerebral arteries are measured postmortem. Acute (6–12 h) and persistent (1–3 days) phases of vascular narrowing are observed in cerebral arteries. Vascular narrowing is associated with thickening of the vascular wall and corrugation of the internal elastic lamina; these histological findings have been described by Lin and associates.1 A key advantage of this model is the use of a species for which a variety of resources are available, such as a range of genetically-modified animals. This mouse model could therefore assist in identifying and verifying mechanisms contributing to vascular pathology associated with SAH and facilitate the initial screening of candidate therapies for cerebral vasospasm.

Keywords

Subarachnoid hemorrhage Cerebral vasospasm Mouse Vasoconstriction Stroke 

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References

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Copyright information

© Humana Press, a part of Springer Science + Business Media, LLC 2009

Authors and Affiliations

  • Tarkan Calisaneller
    • 1
    • 2
  • Chih-Lung Lin
    • 1
    • 3
  • Naoya Ukita
    • 1
    • 4
  • Ryo Yoshimura
    • 1
    • 4
  • Yi Wang
    • 1
    • 5
  • Mark Fitzgerald
    • 1
  • Kevin S. Lee
    • 1
    • 6
  1. 1.Department of NeuroscienceUniversity of Virginia Health SystemCharlottesvilleUSA
  2. 2.Neurosurgery DepartmentBaskent University Faculty of MedicineAnkaraTurkey
  3. 3.Department of NeurosurgeryKaohsiung Medical UniversityKaohsiungTaiwan
  4. 4.Department of Neurological SurgeryOkayama University School of MedicineOkayamaJapan
  5. 5.Department of Neurosurgery, Rui Jin HospitalShanghai Jiao Tong University School of MedicineShanghai
  6. 6.Department of Neurological SurgeryUniversity of VirginiaCharlottesvilleUSA

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