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Targeted Occlusion to Surface and Deep Vessels in Neocortex via Linear and Nonlinear Optical Absorption

  • David Kleinfeld
  • Beth Friedman
  • Patrick D. Lyden
  • Andy  Y. Shih
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Part of the Springer Protocols Handbooks book series (SPH)

We discuss two complementary methods for the study of cerebral blood flow and brain function in response to the occlusion of individual, targeted blood vessels. These bear on the study of microstroke and vascular dysfunction in cortex. One method makes use of linear optical absorption by a photosensitizer, transiently circulated in the blood stream, to induce an occlusion in a surface or near-surface vessel. The second method makes use of nonlinear optical interactions, without the need to introduce an exogenous absorber, to induce an occlusion in a subsurface microvessel. A feature of both methods is that the dynamics of blood flow and functional aspects of the vas-culature and underlying neurons in the neighborhood of the occluded vessel may be monitored before, during, and after the occlusion. We present details of both methods and associated surgical procedures, along with exemplary data from published studies.

Keywords

Ablation Clot Hypoxia Ischemia Microstroke Photosensitizer Plasma Rodent Two-photon 

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Copyright information

© Humana Press, a part of Springer Science + Business Media, LLC 2009

Authors and Affiliations

  • David Kleinfeld
    • 1
  • Beth Friedman
    • 1
  • Patrick D. Lyden
    • 1
  • Andy  Y. Shih
    • 2
  1. 1.Department of Physics, Graduate Program in NeurosciencesUniversity of California at San DiegoLa JollaUSA
  2. 2.Neuroprotection Research Laboratory, Departments of Neurology and Radiology, Massachusetts General HospitalHarvard Medical SchoolCharlestownUSA

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