Transient Global Cerebral Ischemia Model in Mice

  • Kuniyasu Niizuma
  • Hidenori Endo
  • Chikako Nito
  • D. Jeannie Myer
  • Gab Seok Kim
  • Teiji Tominaga
  • Pak H. Chan
Part of the Springer Protocols Handbooks book series (SPH)

The transient global ischemia model is used to analyze selective neuronal death in vulnerable regions including the CA1 subregion of the hippocampus and in certain cortical neurons. In this mouse global ischemia model, bilateral common carotid arteries are reversibly occluded, and the individual anatomical backgrounds are normalized by evaluating the patency of the posterior communicating artery. Using this evaluation, this model might be used for mouse strains with various genetic backgrounds.


Blood flow Delayed neuronal death Global cerebral ischemia model Hippocampus Ischemic brain injury Mice 


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Copyright information

© Humana Press, a part of Springer Science + Business Media, LLC 2009

Authors and Affiliations

  • Kuniyasu Niizuma
    • 1
  • Hidenori Endo
    • 1
    • 2
  • Chikako Nito
    • 1
  • D. Jeannie Myer
    • 1
  • Gab Seok Kim
    • 1
  • Teiji Tominaga
    • 2
  • Pak H. Chan
    • 1
  1. 1.Department of Neurosurgery, Department of Neurology and Neurological Sciences, and Program in NeurosciencesStanford University School of MedicineStanfordUSA
  2. 2.Department of NeurosurgeryTohoku University Graduate School of MedicineSendaiJapan

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