Mesenchymal Stem Cells pp 69-79

Part of the Methods in Molecular Biology™ book series (MIMB, volume 449)

Isolation of Human Adipose-derived Stem Cells from Biopsies and Liposuction Specimens

  • Severine G. Dubois
  • Elizabeth Z. Floyd
  • Sanjin Zvonic
  • Gail Kilroy
  • Xiying Wu
  • Stacy Carling
  • Yuan Di C. Halvorsen
  • Eric Ravussin
  • Jeffrey M. Gimble

Abstract

Adipose tissue has proven to serve as an abundant, accessible, and rich source of adult stem cells with multipotent properties suitable for tissue engineering and regenerative medical applications. Here, we describe a detailed method for the isolation and expansion of adipose-derived stem cells (ASCs). We present a large scale procedure suitable for processing >100 mL volumes of lipoaspirate tissue specimens and a small scale procedure suitable for processing adipose tissue biopsy specimens of < 0.5 g. Although we have focused on the isolation of ASCs from human adipose tissue, the procedure can be applied to adipose tissues from other species with minimal modifications.

Keywords

Adipose-derived stem cells (ASCs) biopsy expansion human isolation lipoaspirate mesenchymal stem cells (MSCs) stromal vascular fraction (SVF) 

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Copyright information

© Humana Press, a part of Springer Science+Business Media, LLC 2008

Authors and Affiliations

  • Severine G. Dubois
    • 1
  • Elizabeth Z. Floyd
    • 1
  • Sanjin Zvonic
    • 1
  • Gail Kilroy
    • 1
  • Xiying Wu
    • 1
  • Stacy Carling
    • 1
  • Yuan Di C. Halvorsen
    • 2
  • Eric Ravussin
    • 1
  • Jeffrey M. Gimble
    • 1
  1. 1.Pennington Biomedical Research CenterBaton RougeLA
  2. 2.Curagen Corp.BranfordCT

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