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Isolation of Phage via Induction of Lysogens

  • Ra’l R. Raya
  • Elvira M. H’bert
Part of the Methods in Molecular Biology™ book series (MIMB, volume 501)

Abstract

Most bacterial cells carry prophage genomes either integrated into the host DNA or present as repressed plasmids. Methods are described for the induction of prophages using Mitomycin C, and for the isolation of prophage-cured bacterial cell lines.

Key Words

Lysogen induction lytic cycle lysogenic cycle prophage integration SOS response mitomycin RecA repressor 

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Copyright information

© Humana Press, a part of Springer Science+Business Media, LLC 2009

Authors and Affiliations

  • Ra’l R. Raya
    • 1
  • Elvira M. H’bert
    • 1
  1. 1.Cerela-Conicet, S.M. de TucumáArgentina

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