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Bacteriophage Enrichment from Water and Soil

  • Rohan Van Twest
  • Andrew M. Kropinski
Part of the Methods in Molecular Biology™ book series (MIMB, volume 501)

Abstract

Classical bacterial enrichment devised by Sergius Winogradsky (1856–1953) and Martinus Beijerinck (1851–1931) can be modified to enrich for bacteria-specific viruses. In this chapter simple protocols are presented for the enrichment of phages from water samples, such as sewage, and soil.

Key Words

Enrichment soil sewage Bacillus Pseudomonas enterics enrichment bias membrane filtration water 

References

  1. 1.
    Daubin, V. and H. Ochman. 2004. Bacterial genomes as new gene homes: the genealogy of ORFans in E. coli. Genome Research 14:1036–1042.CrossRefPubMedGoogle Scholar
  2. 2.
    Miao, E.A. and S.I. Miller. 1999. Bacteriophages in the evolution of pathogen-host interactions. Proceedings of the National Academy of Sciences of the United States of America 96:9452–9454.CrossRefPubMedGoogle Scholar
  3. 3.
    Ai, Y., F. Meng, and Y. Zeng. 2000. The evolution of pathogen-host interactions mediated by bacteriophages. Wei Sheng Wu Hsueh Pao – Acta Microbiologica Sinica 40: 657–660.Google Scholar
  4. 4.
    Dobrindt, U. and J. Reidl. 2000. Pathogenicity islands and phage conversion: evolutionary aspects of bacterial pathogenesis. Ijmm International Journal of Medical Microbiology 290:519–527.Google Scholar
  5. 5.
    Canchaya, C., G. Fournous, S. Chibani-Chennoufi, M.L. Dillmann, and H. Brussow. 2003. Phage as agents of lateral gene transfer. Current Opinion in Microbiology 6: 417–424.CrossRefPubMedGoogle Scholar
  6. 6.
    Sakaguchi, Y., T. Hayashi, K. Kurokawa, K. Nakayama, K. Oshima, Y. Fujinaga, M. Ohnishi, E. Ohtsubo, et al. 2005. The genome sequence of Clostridium botulinum type C neurotoxin-converting phage and the molecular mechanisms of unstable lysogeny. Proceedings of the National Academy of Sciences of the United States of America 102: 17472–17477.CrossRefPubMedGoogle Scholar
  7. 7.
    Williamson, K.E., M. Radosevich, and K.E. Wommack. 2005. Abundance and diversity of viruses in six Delaware soils. Applied & Environmental Microbiology 71:3119–3125.CrossRefGoogle Scholar
  8. 8.
    Williamson, K.E., K.E. Wommack, M. Radosevich, K.E. Williamson, K.E. Wommack, and M. Radosevich. 2003. Sampling natural viral communities from soil for culture-independent analyses. Applied & Environmental Microbiology 69:6628–6633.CrossRefGoogle Scholar
  9. 9.
    Rohwer, F. 2003. Global phage diversity. Cell 113:141.CrossRefPubMedGoogle Scholar
  10. 10.
    Breitbart, M., L. Wegley, S. Leeds, T. Schoenfeld, and F. Rohwer. 2004. Phage community dynamics in hot springs. Applied & Environmental Microbiology 70: 1633–1640.CrossRefGoogle Scholar
  11. 11.
    Filee, J., F. Tetart, C.A. Suttle, H.M. Krisch, J. Filee, F. Tetart, C.A. Suttle, and H.M. Krisch. 2005. Marine T4-type bacteriophages, a ubiquitous component of the dark matter of the biosphere. Proceedings of the National Academy of Sciences of the United States of America 102:12471–12476.CrossRefPubMedGoogle Scholar
  12. 12.
    Kepner, R.L., Jr., R.A. Wharton, Jr., and C.A. Suttle. 1998. Viruses in Antarctic lakes. Limnology & Oceanography 43:1754–1761.CrossRefGoogle Scholar
  13. 13.
    Paul, J.H., J.B. Rose, S.C. Jiang, C.A. Kellogg, L. Dickson, J.H. Paul, J.B. Rose, S.C. Jiang, et al. 1993. Distribution of viral abundance in the reef environment of Key Largo, Florida. Applied & Environmental Microbiology 59:718–724.Google Scholar
  14. 14.
    Dunbar, J., S. White, and L. Forney. 1997. Genetic diversity through the looking glass: Effect of enrichment bias. Applied & Environmental Microbiology 63:1326–1331.Google Scholar
  15. 15.
    Mendez, J., A. Audicana, M. Cancer, A. Isern, J. Llaneza, B. Moreno, M. Navarro, M.L. Tarancon, et al. 2004. Assessment of drinking water quality using indicator bacteria and bacteriophages. Journal of Water & Health 2:201–214.Google Scholar
  16. 16.
    Mendez, J., A. Audicana, A. Isern, J. Llaneza, B. Moreno, M.L. Tarancon, J. Jofre, F. Lucena, et al. 2004. Standardised evaluation of the performance of a simple membrane filtration-elution method to concentrate bacteriophages from drinking water. Journal of Virological Methods 117:19–25.CrossRefPubMedGoogle Scholar
  17. 17.
    Lucena, F., F. Ribas, A.E. Duran, S. Skraber, C. Gantzer, C. Campos, A. Moron, E. Calderon, et al. 2006. Occurrence of bacterial indicators and bacteriophages infecting enteric bacteria in groundwater in different geographical areas. Journal of Applied Microbiology 101:96–102.CrossRefPubMedGoogle Scholar
  18. 18.
    Haberer, K. and J. Maniloff. 1982. Adsorption of the tailed mycoplasma virus L3 to cell membranes. Journal of Virology 41:501–507.PubMedGoogle Scholar
  19. 19.
    Landry, E.F. and R.M. Zsigray. 1980. Effects of calcium on the lytic cycle of Bacillus subtilis phage 41c. Journal of General Virology 51:125–135.CrossRefPubMedGoogle Scholar
  20. 20.
    Mahony, D.E., P.D. Bell, and K.B. Easterbrook. 1985. Two bacteriophages of Clostridium difficile. Journal of Clinical Microbiology 21:251–254.PubMedGoogle Scholar
  21. 21.
    Ashelford, K.E., M.J. Day, and J.C. Fry. 2003. Elevated abundance of bacteriophage infecting bacteria in soil. Applied & Environmental Microbiology 69:285–289.CrossRefGoogle Scholar
  22. 22.
    Danovaro, R., E. Manini, and A. Dell’Anno. 2002. Higher abundance of bacteria than viruses in deep Mediterranean sediment. Applied & Environmental Microbiology 68:1468–1472.CrossRefGoogle Scholar
  23. 23.
    Danovaro, R., A. Dell’Anno, M. Serresi, and S. Vanucci. 2001. Determination of virus adundance in marine sediments. Applied & Environmental Microbiology 67:1384–1387.CrossRefGoogle Scholar

Copyright information

© Humana Press, a part of Springer Science+Business Media, LLC 2009

Authors and Affiliations

  • Rohan Van Twest
    • 1
  • Andrew M. Kropinski
    • 1
    • 2
    • 3
  1. 1.Department of Molecular and Cellular BiologyUniversity of GuelphGuelphCanada
  2. 2.Public Health Agency of CanadaLaboratory for Foodborne DiseasesGuelphCanada
  3. 3.Department of Microbiology and ImmunologyQueen’s UniversityKingstonCanada

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