Bacteriophage Enrichment from Water and Soil

  • Rohan Van Twest
  • Andrew M. Kropinski
Part of the Methods in Molecular Biology™ book series (MIMB, volume 501)


Classical bacterial enrichment devised by Sergius Winogradsky (1856–1953) and Martinus Beijerinck (1851–1931) can be modified to enrich for bacteria-specific viruses. In this chapter simple protocols are presented for the enrichment of phages from water samples, such as sewage, and soil.

Key Words

Enrichment soil sewage Bacillus Pseudomonas enterics enrichment bias membrane filtration water 


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Copyright information

© Humana Press, a part of Springer Science+Business Media, LLC 2009

Authors and Affiliations

  • Rohan Van Twest
    • 1
  • Andrew M. Kropinski
    • 1
    • 2
    • 3
  1. 1.Department of Molecular and Cellular BiologyUniversity of GuelphGuelphCanada
  2. 2.Public Health Agency of CanadaLaboratory for Foodborne DiseasesGuelphCanada
  3. 3.Department of Microbiology and ImmunologyQueen’s UniversityKingstonCanada

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