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Large-Scale Gene Disruption Using the UAU1 Cassette

  • Clarissa J. Nobile
  • Aaron P. Mitchell
Part of the Methods in Molecular Biology book series (MIMB, volume 499)

Abstract

Candida albicans is a major fungal systemic pathogen in humans. Genetic manipulation of C. albicans is unwieldy. We report here a strategy that is useful and successful for large-scale genetic manipulation of C. albicans genes of interest: use of the UAU1 cassette on a Tn7 transposon. Streamlined yet admittedly flawed disruption techniques, such as the one described here, may prove vital to uncovering the genetic basis of fungal virulence.

Key words

UAU1 large-scale gene disruption mutants 

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Copyright information

© Humana Press, a part of Springer Science+Business Media, LLC 2009

Authors and Affiliations

  • Clarissa J. Nobile
    • 1
  • Aaron P. Mitchell
  1. 1.Department of Microbiology Columbia UniversityNew York

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