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Micropropagation of Poinsettia by Organogenesis

  • Marcos Castellanos
  • J. Brian Power
  • Michael R. DaveyEmail author
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 589)

Abstract

Poinsettia (Euphorbia pulcherrima) is one of the most popular ornamental pot plants. Conventional propagation is by cuttings, generally focused on a period prior to the most intensive time of sales. Rapid multiplication of elite clones, the production of pathogen-free plants and more rapid introduction of novel cultivars (cvs.) with desirable traits, represent important driving forces in the poinsettia industry. In recent years, different strategies have been adopted to micropropagate poinsettia, which could assist breeders to meet consumer demands. The development of reliable in vitro regeneration procedures is likely to play a crucial role in future production systems. Stem nodal explants cultured on semi-solid MS-based medium supplemented with benzylaminopurine (BAP) and naphthalene acetic acid (NAA) develop shoots from adventitious/axillary buds after 7 weeks of culture. Rooting of in vitro regenerated shoots is achieved in semi-solid MS-based medium containing the auxin indole-3-acetic acid (IAA). Four to six weeks after transfer to root-inducing medium, regenerated plants can be transferred to compost and acclimatized in the glasshouse. Direct shoot regeneration from cultured explants is important to minimize somaclonal variation in regenerated plants.

Key words:

Euphorbia pulcherrima Ornamental plants Shoot regeneration Stem nodal explants Tissue culture 

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Copyright information

© Humana Press, a part of Springer Science+Business Media, LLC 2010

Authors and Affiliations

  • Marcos Castellanos
    • 1
  • J. Brian Power
    • 1
  • Michael R. Davey
    • 1
    Email author
  1. 1.Plant and Crop Sciences Division, School of BiosciencesUniversity of NottinghamLoughboroughUK

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