A Highly Efficient Protocol for Micropropagation of Begonia tuberous

  • Duong Tan Nhut
  • Nguyen Thanh Hai
  • Mai Xuan Phan
Protocol
First Online:
Part of the Methods in Molecular Biology book series (MIMB, volume 589)

Abstract

A protocol for micropropagation of begonia was established utilizing a thin cell layer (TCL) system. This system has been employed to produce several thousand shoots per sample. Explant size and position, and plant growth regulators (PGRs) contribute to the tissue morphogenesis. By optimizing the size of the tissue and applying an improved selection procedure, shoots were elongated in 8 weeks of culture, with an average number of 210 ± 9.7 shoots per segment. This system has facilitated a number of studies using TCL as a model for micropropagation and will enable the large-scale production of begonia. On an average, the best treatment would allow production of about 10,000 plantlets by the micropropagation of the axillary buds of one plant with five petioles, within a period of 8 months.

Key words

Begonia Thin cell layer Micropropagation TDZ Petiole Stem Floral stalk 
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Copyright information

© Humana Press, a part of Springer Science+Business Media, LLC 2010

Authors and Affiliations

  • Duong Tan Nhut
    • 1
  • Nguyen Thanh Hai
    • 1
  • Mai Xuan Phan
    • 1
  1. 1.Plant Molecular Biology and Plant Breeding LaboratoryTay Nguyen Institute of BiologyDalatVietnam

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