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Transgenic Delivery and Detection of GFP in Neuropeptide Neurons

  • J.L. Holter
  • J.S. Davies
  • P.-S. Man
  • T. Wells
  • D.A. Carter
Part of the Neuromethods book series (NM, volume 39)

Introduction

The first bioengineered autofluorescent protein, GFP (green fluorescent protein), was developed more than 10 years ago (1). In recent years both this fluorescent protein and numerous variants (alternatively colored, destabilized, etc.; see (2)) have been widely adopted in experimental neuroscience (3). When expressed in cultured cells and transgenic animals, GFP provides for direct (in situ) cellular visualization and thereby optical selection of individual cells for morphological, physiological, and molecular characterization. For some studies, generalized expression of GFP (e.g., actin gene promoter-driven; (4)) may be useful in facilitating the analysis of individual cell types. In general, however, the principal value of FP expression lies in spatial resolution, obtained through restriction of expression to a subpopulation of cells. In this case a more refined transgenic approach is required in which GFP is expressed under the control of a cell-specific gene promoter....

Key Words

transgene rat fluorescent protein immunocytochemistry Western blot somatostatin pituitary cortex striatum 

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Copyright information

© Humana Press Inc. 2008

Authors and Affiliations

  • J.L. Holter
    • 1
  • J.S. Davies
    • 2
  • P.-S. Man
    • 2
  • T. Wells
    • 2
  • D.A. Carter
    • 2
  1. 1.Department of Basic Sciences and Aquatic MedicineThe Norwegian School of Veterinary Science, UllevålsveienOsloNorway
  2. 2.School of BiosciencesCardiff UniversityCardiffUK

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