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Post-translational Modifications of Proteins pp 95–108Cite as

Lysine Hydroxylation and Cross-linking of Collagen

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Part of the Methods in Molecular Biology™ book series (MIMB,volume 446)

Summary

Collagens represent a large family of structurally related extracellular matrix proteins containing unique triple helical structure. One of the characteristics of this structural protein is its extensive post-translational modifications that have major effects on molecular assembly, stability, and metabolism. Hydroxylation of specific lysine residues is one of such unique modifications found in collagen, and the pattern/extent of this modification influences fibrillogenesis, cross-linking, and matrix mineralization. The formation of covalent intermolecular cross-linking is the final modification in collagen biosynthesis and is critical for the stability of collagen. The process of cross-linking is dynamic and the pathways vary depending on the tissues and tissue's physiological state. This tissue specificity of cross-linking pattern may in part be the results of differential expression of various isoforms of lysyl hydroxylases and lysyl oxidases that have been recently identified and partially characterized. This chapter concentrates on recent research progress on these two modifications and the methods for analysis we have developed.

Key Words

  • Collagen post-translational modifications
  • collagen cross-linking
  • lysine hydroxylation
  • amino acid analysis

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Acknowledgments

This study was supported by NIH grants DE10489, AR052824 and NASA grant NAG2-1596.

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Authors and Affiliations

  1. Dental Research Center, University of North Carolina, Chapel Hill, NC, USA

    Mitsuo Yamauchi & Masashi Shiiba

Authors
  1. Mitsuo Yamauchi
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  2. Masashi Shiiba
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Editors and Affiliations

  1. Molecular Biochemistry Department, Octapharma Research and Development, Berlin, Germany

    Christoph Kannicht

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Yamauchi, M., Shiiba, M. (2008). Lysine Hydroxylation and Cross-linking of Collagen. In: Kannicht, C. (eds) Post-translational Modifications of Proteins. Methods in Molecular Biology™, vol 446. Humana Press. https://doi.org/10.1007/978-1-60327-084-7_7

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  • DOI: https://doi.org/10.1007/978-1-60327-084-7_7

  • Publisher Name: Humana Press

  • Print ISBN: 978-1-58829-719-8

  • Online ISBN: 978-1-60327-084-7

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