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Glycoprotein Enrichment Through Lectin Affinity Techniques

  • Yehia Mechref
  • Milan Madera
  • Milos V Novotny
Protocol
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Part of the Methods in Molecular Biology™ book series (MIMB, volume 424)

Summary

Posttranslational modifications (PTM) of proteins are among the key biological regulators of function, activity, localization, and interaction. The fact that no more than 30,000–50,000 proteins are encoded by the human genome underlines the importance of posttranslational modifications in modulating the activities and functions of proteins in health and disease. With approximately 50% of all proteins now considered to be glycosylated, its physiological importance in mammalian systems is imperative. Aberrant glycosylation has now been recognized as an attribute of many mammalian diseases, including hereditary disorders, immune deficiencies, neurodegenerative diseases, cardiovascular conditions, and cancer. As many potential disease biomarkers may be glycoproteins present in only minute quantities in tissue extracts and physiological fluids, glycoprotein isolation and enrichment may be critical in a search for such biomarkers. For decades, efforts have been focused on the development of glycoprotein enrichment from complex biological samples. Logically, the great majority of these enrichment methodologies rely on the use of immobilized lectins, which permit selective enrichment of the pools of glycoproteins for proteomic/glycomic studies. In this chapter, lectin affinity chromatography in different formats are described, including tubes; packed columns, and microfluidic channels.

Key Words

Agarose lectin material glycoprotein enrichment human blood serum glycoproteins lectin affinity chromatography silica-based lectin material 

Notes

Acknowledgements

This work was supported by Grant No. GM24349 from the National Institute of General Medical Sciences, U.S. Department of Health and Human Services and a center grant from the Indiana 21st Century Research and Technology Fund. This work was also supported by the National Institute of Health through the National Center for Research Resources (NCRR) by grant number RR018942 for the National Center for Glycomics and Glycoproteomics.

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Copyright information

© Humana Press, a part of Springer Science+Business Media, LLC 2008

Authors and Affiliations

  • Yehia Mechref
    • 1
  • Milan Madera
    • 1
  • Milos V Novotny
    • 1
  1. 1.Indiana UniversityBloomingtonIndiana

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