Isolation of Phosphoproteins

  • Lawrence G. Puente
  • Lynn A. Megeney
Part of the Methods in Molecular Biology™ book series (MIMB, volume 424)

Summary

Phosphorylation is one of the most abundant post-translational modifications on protein and one that frequently has functional biological consequences. For this reason, screening protein samples for phosphorylations has become an important tool in biochemical research. Affinity purification by immunological or chemical reagents can be used to isolate phosphoproteins from other cellular materials.

Key Words

Affinity purification immunoprecipitation protein phosphorylation 

References

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Copyright information

© Humana Press, a part of Springer Science+Business Media, LLC 2008

Authors and Affiliations

  • Lawrence G. Puente
    • 1
  • Lynn A. Megeney
    • 1
  1. 1.The Ottawa HospitalOttawaCanada

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