2D PAGE: Sample Preparation and Fractionation pp 23-34

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Bacteria and Yeast Cell Disruption Using Lytic Enzymes

  • Oriana Salazar

Summary

Enzymatic methods provide a convenient alternative for overcoming technical disadvantages of mechanical disruption. Protocols for protein extraction from bacteria and Saccharomyces cerevisiae using lytic enzymes are presented in this chapter. Adaptation of the yeast protocol to a microtiter plate format makes this protocol amenable for proteomic applications and high-throughput screening of libraries expressing genetic variants in yeast. This methodology can also be applied to bacteria.

Key Words

Cell lysis yeast-lytic endoglucanase bacteriolytic enzyme lysozyme microtiter-plate format 

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Copyright information

© Humana Press, a part of Springer Science+Business Media, LLC 2008

Authors and Affiliations

  • Oriana Salazar
    • 1
  1. 1.University of ChileSantiagoChile

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