Structural Proteomics pp 537-559

Part of the Methods in Molecular Biology™ book series (MIMB, volume 426)

Bacterial Structural Genomics Initiative: Overview of Methods and Technologies Applied to the Process of Structure Determination

  • Miroslaw Cygler
  • Ming-ni Hung
  • John Wagner
  • Allan Matte

The focus over the last several years on increasing the number of three-dimensional structures of macromolecules by implementation of high throughput methodology has led to the establishment of dedicated structural genomics programs around the world. These worldwide efforts have in turn led to development of novel, parallelized approaches to cloning, expression, purification, and crystallization of proteins. This chapter describes in some detail the approaches and protocols that have been implemented in the Bacterial Structural Genomics Initiative.


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Copyright information

© Humana Press, a part of Springer Science+Business Media, LLC 2008

Authors and Affiliations

  • Miroslaw Cygler
    • 1
  • Ming-ni Hung
    • 1
  • John Wagner
    • 1
  • Allan Matte
    • 1
  1. 1.Biotechnology Research InstituteNational Research Council CanadaMontrealCanada

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