Transgenesis Techniques pp 185-197

Part of the Methods in Molecular Biology book series (MIMB, volume 561) | Cite as

Targeting Embryonic Stem Cells

Protocol

Summary

Gene targeting of mouse embryonic stem cells facilitates the deliberate engineering of the mouse genome. The gene targeting vector is introduced into cells by electroporation, and cell clones carrying the targeting vector are obtained by drug selection. Clones can be screened effectively for correct recombination events by either Southern blot analysis with external and internal probes, or by long-range PCR. Positive clones are subsequently expanded and prepared for the generation of chimeric mice.

Key words

Gene Targeting ES cells Southern blot analysis Long-range PCR 

References

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Copyright information

© Humana Press, a part of Springer Science+Business Media, LLC 2009

Authors and Affiliations

  1. 1.Helmholtz Center MunichInstitute of Developmental GeneticsNeuherbergGermany

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