Apoptosis pp 343-356

Part of the Methods in Molecular Biology book series (MIMB, volume 559)

Detection of Cell Death in Drosophila

  • Kimberly McCall
  • Jeanne S. Peterson
  • Tracy L. Pritchett
Protocol

Summary

Drosophila is a powerful model system for the identification of cell death genes and understanding the role of cell death in development. In this chapter, we describe three methods typically used for the detection of cell death in Drosophila. The TUNEL and acridine orange methods are used to detect dead or dying cells in a variety of tissues. We focus on methods for the embryo and the ovary, but these techniques can be used on other tissues as well. The third method is the detection of genetic interactions by expressing cell death genes in the Drosophila eye.

Key words

Drosophila Apoptosis Cell death Embryo Ovary 

Copyright information

© Humana Press, a part of Springer Science+Business Media, LLC 2009

Authors and Affiliations

  • Kimberly McCall
    • 1
  • Jeanne S. Peterson
    • 1
  • Tracy L. Pritchett
    • 1
  1. 1.Department of BiologyBoston UniversityBostonUSA

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