Purification of Arabidopsis Argonaute Complexes and Associated Small RNAs

  • Yijun Qi
  • Shijun Mi
Protocol
First Online:
Part of the Methods in Molecular Biology book series (MIMB, volume 592)

Abstract

Argonaute (AGO) proteins recruit small RNAs to form effector complexes of RNA interference (RNAi), collectively termed RNA-induced silencing complexes (RISCs). Here, we describe detailed protocols for the purification of AGO complexes and their associated small RNAs, using Arabidopsis AGO1 as an example.

Key words

RNAi Argonaute microRNA Purification 
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Notes

Acknowledgments

This work was supported by the Ministry of Science and Technology of China.

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Copyright information

© Humana Press, a part of Springer Science+Business Media, LLC 2010

Authors and Affiliations

  • Yijun Qi
    • 1
  • Shijun Mi
    • 1
  1. 1.National Institute of Biological SciencesBeijingChina

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