Drosophila pp 289-302 | Cite as

Fluorescent In Situ Hybridization Protocols in Drosophila Embryos and Tissues

  • Eric Lécuyer
  • Neela Parthasarathy
  • Henry M. Krause
Part of the Methods in Molecular Biology book series (MIMB, volume 420)


Fluorescent in situ hybridization is the standard method for visualizing the spatial distribution of RNA. Although traditional histochemical RNA detection methods suffered from limitations in resolution or sensitivity, the recent development of peroxidase-mediated tyramide signal amplification provides strikingly enhanced sensitivity and subcellular resolution. In this chapter, we describe optimized fluorescent in situ hybridization protocols for Drosophila embryos and tissues utilizing tyramide signal amplification, either for single genes or in a high-throughput format, which greatly increases the sensitivity, consistency, economy, and throughput of the procedure. We also describe variations of the method for RNA-RNA and RNA-protein codetection.

Key Words

Drosophila melanogaster embryos and tissues FISH fluorescent in situ hybridization RNA-protein costaining single and double labeling tyramide signal amplification 


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Copyright information

© Humana Press Inc., Totowa, NJ 2008

Authors and Affiliations

  • Eric Lécuyer
    • 1
  • Neela Parthasarathy
    • 1
  • Henry M. Krause
    • 1
  1. 1.Donnelly Centre for Cellular and Biomolecular ResearchUniversity of TorontoTorontoCanada

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