Drosophila pp 265-275 | Cite as

Imaging Drosophila Pupal Wing Morphogenesis

Part of the Methods in Molecular Biology book series (MIMB, volume 420)


Drosophila pupal (P) wing development entails a series of dynamic developmental events, such as epithelial and glial morphogenesis, that are of outstanding interest to cell biologists. Here, we first describe how to prepare P and prepupal (PP) wings for immunofluorescence microscopy. This protocol has been optimized to visualize wing epithelial architecture, such as polarized cortical domains of planar cell polarity proteins. We then provide a protocol to prepare pupae for whole mount live imaging of P wings. This procedure has allowed us to live-image glial cell migration and proliferation along wing sensory nerves.

Key Words

Drosophila glial cell migration immunofluorescence microscopy live imaging planar cell polarity pupal wing 


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Copyright information

© Humana Press Inc., Totowa, NJ 2008

Authors and Affiliations

  1. 1.Department of Molecular and Cell BiologyUniversity of CaliforniaBerkeleyUSA
  2. 2.Max Planck Institute of Molecular Cell Biology and GeneticsDresdenGermany
  3. 3.Institut de Genetique et de Biologie Moleculaire et Cellulaire CNRS/INSERM/ULP, Illkirch CedexCU de StrasbourgFrance

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