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Drosophila pp 265-275 | Cite as

Imaging Drosophila Pupal Wing Morphogenesis

  • Anne-Kathrin Classen
  • Benoit Aigouy
  • Angela Giangrande
  • Suzanne Eaton
Part of the Methods in Molecular Biology book series (MIMB, volume 420)

Abstract

Drosophila pupal (P) wing development entails a series of dynamic developmental events, such as epithelial and glial morphogenesis, that are of outstanding interest to cell biologists. Here, we first describe how to prepare P and prepupal (PP) wings for immunofluorescence microscopy. This protocol has been optimized to visualize wing epithelial architecture, such as polarized cortical domains of planar cell polarity proteins. We then provide a protocol to prepare pupae for whole mount live imaging of P wings. This procedure has allowed us to live-image glial cell migration and proliferation along wing sensory nerves.

Key Words

Drosophila glial cell migration immunofluorescence microscopy live imaging planar cell polarity pupal wing 

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Copyright information

© Humana Press Inc., Totowa, NJ 2008

Authors and Affiliations

  • Anne-Kathrin Classen
    • 1
  • Benoit Aigouy
    • 2
  • Angela Giangrande
    • 3
  • Suzanne Eaton
    • 2
  1. 1.Department of Molecular and Cell BiologyUniversity of CaliforniaBerkeleyUSA
  2. 2.Max Planck Institute of Molecular Cell Biology and GeneticsDresdenGermany
  3. 3.Institut de Genetique et de Biologie Moleculaire et Cellulaire CNRS/INSERM/ULP, Illkirch CedexCU de StrasbourgFrance

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