Drosophila pp 175-195 | Cite as

Recombinases and Their Use in Gene Activation, Gene Inactivation, and Transgenesis

  • Johannes Bischof
  • Konrad Basler
Part of the Methods in Molecular Biology book series (MIMB, volume 420)


The site-specific recombinase FLP is used in Drosophila to precisely manipulate the genome, in particular, to eliminate gene function by mitotic recombination and to activate transgenes in discrete populations of cells. These approaches are already part of the standard tool kit for studying gene function. The number of applications for the FLP recombinase has increased over the years and further members of the large family of site-specific recombinases are being added to the arsenal of fly geneticists, most recently, the φC31 integrase. This chapter will introduce these recombinases and describe how such instruments are utilized to accurately manipulate the Drosophila genome.

Key Words

Drosophila melanogaster Cre/loxP FLP/FRT φC31 integrase mitotic recombination mosaics recombinases transgenesis 


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Copyright information

© Humana Press Inc., Totowa, NJ 2008

Authors and Affiliations

  • Johannes Bischof
    • 1
  • Konrad Basler
    • 1
  1. 1.Institute of Molecular BiologyUniversity of ZürichZürichSwitzerland

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