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FISH for Mapping Single Copy Genes

  • Terje Raudsepp
  • Bhanu P. Chowdhary
Protocol
Part of the Methods in Molecular Biology™ book series (MIMB, volume 422)

Abstract

During the past two decades fluorescent in-situ hybridization (FISH) has become a standard technique to directly localize, orient, and order genes in the genomes of a wide range of species. Despite the availability of a variety of probes, probe labeling and signal-detection systems, and advanced image analysis software, the core procedures used to carry out FISH remain the same. A detailed overview of these procedures, including target preparation (metaphase/interphase chromosomes and DNA fibers), probe labeling, in-situ hybridization, signal detection, and imaging, is here provided in a stepwise manner.

Key Words

FISH gene mapping metaphase chromosomes interphase chromosomes DNA fibers DNA labeling 

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Copyright information

© Humana Press Inc., Totowa, NJ 2008

Authors and Affiliations

  • Terje Raudsepp
    • 1
  • Bhanu P. Chowdhary
    • 1
  1. 1.Department of Veterinary Integrative BiosciencesTexas A&M UniversityCollege Station

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