Intravital Two-Photon Imaging of Natural Killer Cells and Dendritic Cells in Lymph Nodes

  • Susanna Celli
  • Béatrice Breart
  • Philippe Bousso
Part of the Methods in Molecular Biology™ book series (MIMB, volume 415)


Two-photon microscopy makes it possible to image in real-time fluorescently labeled cells located in deep tissue environments. We describe a procedure to visualize the behavior of natural killer (NK) cells and dendritic cells (DC) in the lymph nodes of live, anesthetized mice. Intravital two-photon imaging is a powerful tool to study the migration and cell interactions of immune cells such as NK and DC in physiological settings.

Key Words

NK cells dendritic cells lymph node intravital imaging two-photon microscopy 


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Copyright information

© Humana Press Inc. 2008

Authors and Affiliations

  • Susanna Celli
    • 1
  • Béatrice Breart
    • 1
  • Philippe Bousso
    • 1
  1. 1.Département d’ImmunologieG5 Dynamiques des Réponses Immunes, Equipe AvenirInstitut Pasteur, Paris CedexFrance

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