KIR Genotyping and Analysis

Genotyping and Disease Association Analysis
  • Maureen P. Martin
  • Mary Carrington
Part of the Methods in Molecular Biology™ book series (MIMB, volume 415)

Summary

The genes encoding the killer immunoglobulin-like receptors (KIR) are situated within a segment of DNA that has undergone expansion and contraction over time due in large part to unequal crossing over. Consequently, individuals exhibit considerable haplotypic variation in terms of gene content. The highly polymorphic human leukocyte antigen (HLA) class I loci encode ligands for the KIR; thus, it is not surprising that KIR genes also show significant allelic polymorphism. As a result of the receptor–ligand relationship between KIR and HLA, functionally relevant KIR–HLA combinations need to be considered in the analysis of these genes as they relate to disease outcomes. This chapter will describe a genotyping method for identifying the presence/absence of the KIR genes and general approaches to data analysis in disease association studies.

Keywords

KIR genotyping sequence-specific priming (SSP) natural killer (NK) cells HLA class I ligands KIR haplotypes 

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Copyright information

© Humana Press Inc. 2008

Authors and Affiliations

  • Maureen P. Martin
    • 1
  • Mary Carrington
    • 1
  1. 1.Laboratory of Genomic DiversitySAIC-Frederick, Inc., NCI-FrederickFrederick

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