Tumor-Targeted Salmonella typhimurium Overexpressing Cytosine Deaminase: A Novel, Tumor-Selective Therapy

Protocol
Part of the Methods in Molecular Biology™ book series (MIMB, volume 542)

Summary

The ideal anticancer regimen is one that is specific for cancer cells with limited toxicity to normal tissues. Genetically modified, nonpathogenic Salmonella offer a potential way to induce direct tumoricidal activity or to deliver tumoricidal agents to tumors. An attenuated strain of Salmonella typhimurium, called VNP20009, and its derivative TAPET-CD (which expresses Escherichia coli cytosine deaminase) are highly selective for tumor tissue and can deliver therapeutic proteins preferentially to tumors in preclinical models. Both VNP20009 and TAPET-CD have been investigated successfully in Phase 1 clinical trials in cancer patients.

Key Words

cytosine deaminase 5-fluorocytosine 5-fluorouracil Salmonella TAPET-CD tumor VNP20009 

Notes

Acknowledgements

The authors thank Brooks Low, Samuel Miller, and John Pawelek for helpful suggestions and Kimberly Troy, Jeremy Pike, and Caroline Clairmont for technical assistance. This work was supported by Vion Pharmaceuticals, New Haven, CT.

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Copyright information

© Humana Press, a part of Springer Science+Business Media, LLC 2009

Authors and Affiliations

  1. 1.Vion Pharmaceuticals, Inc.New HavenUSA
  2. 2.Celator Pharmaceuticals Corp.VancouverCanada

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