DNA and RNA Profiling in Human Blood pp 129-142

Part of the METHODS IN MOLECULAR BIOLOGY™ book series (MIMB, volume 496)

Medium- to High-Throughput SNP Genotyping Using VeraCode Microbeads

  • Charles H. Lin
  • Joanne M. Yeakley
  • Timothy K. McDaniel
  • Richard Shen

Abstract

Recent breakthroughs in multiplexed SNP (single nucleotide polymorphism) genotyping technology have enabled global mapping of the relationships between genetic variation and disease. Discoveries made by such whole-genome association studies often spur further interest in surveying more focused subsets of SNPs for validation or research purposes. Here we describe a new SNP genotyping platform that is flexible in assay content and multiplexing (up to 384 analytes), and can serve medium- to high-throughput applications. The Illumina BeadXpress platform supports the GoldenGate Genotyping Assay on digitally inscribed VeraCode microbeads to allow streamlined workflow, rapid detection, unparalleled data reproducibility and consistency. Thus, it is a highly valuable tool for biomarker research and validation, pharmaceutical development, as well as the development of molecular diagnostic tests.

Key Words

High-throughput genotyping biomarker validation SNP randomly assembled microarrays allele-specific extension and ligation universal capture oligonucleotides universal arrays 

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Copyright information

© Humana Press, a part of Springer Science+Business Media, LLC 2009

Authors and Affiliations

  • Charles H. Lin
    • 1
  • Joanne M. Yeakley
    • 1
  • Timothy K. McDaniel
    • 1
  • Richard Shen
    • 1
  1. 1.Illumina, Inc.San DiegoUSA

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