Oligonucleotide Microarrays for Identification of Microbial Pathogens and Detection of Their Virulence-Associated or Drug-Resistance Determinants

  • Dmitriy V. Volokhov
  • Hyesuk Kong
  • Keith Herold
  • Vladimir E. Chizhikov
  • Avraham Rasooly
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 671)

Abstract

Microarrays are spatially ordered arrays with ligands chemically immobilized in discrete spots on a solid matrix, usually a microscope slide. Microarrays are a high-throughput large-scale screening system enabling simultaneous identification of a large number of labeled target molecules (up to several hundred thousand) that bind specifically to the immobilized ligands of the array. DNA microarrays represent a promising tool for clinical, environmental, and industrial microbiology since the technology allows relatively rapid identification of large number of genetic determinants simultaneously, providing detailed genomic level information regarding the pathogen species, including identification of their virulence-associated factors and the presence of antibiotic resistance genes. In this chapter, we describe key aspects and methodologies important for the development and use of DNA microarrays for microbial diagnostics.

Key words

Microarray Microbial pathogens Virulence factors Food safety 

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Copyright information

© Springer Science+Business Media, LLC 2011

Authors and Affiliations

  • Dmitriy V. Volokhov
    • 1
  • Hyesuk Kong
    • 1
  • Keith Herold
    • 2
  • Vladimir E. Chizhikov
    • 1
  • Avraham Rasooly
    • 3
  1. 1.Center for Biologics Evaluation and ResearchFood and Drug AdministrationKensingtonUSA
  2. 2.Department of BioengineeringUniversity of MarylandCollege ParkUSA
  3. 3.National Institutes of Health, National Cancer InstituteFDABethesdaUSA

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