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PDMS Microfluidic Capillary Systems for Patterning Proteins on Surfaces and Performing Miniaturized Immunoassays

  • Mateu Pla-Roca
  • David Juncker
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 671)

Abstract

In this chapter, we describe the fabrication and use of microfluidic capillary systems (CSs) made in soft, transparent polydimethylsiloxane (PDMS). Sixteen microfluidic CSs, each containing a loading pad, a microchannel, and a capillary pump are engraved in a single PDMS chip. The CSs are used for two applications, firstly to pattern fibronectin on glass surfaces to locally control the adhesion of cultured cells to the substrate, and secondly to carry out multiplexed miniaturized immunoassays.

Key words

Microfluidics Miniaturizated immunoassays Micromosaic immunoassays Protein patterning 

Notes

Acknowledgments

The authors would like to thank Emmanuel Delamarche and Ute Drechsler (IBM Research Center, Zurich) for the fabrication of the molds. We are also very grateful to Saravanan Sundararajan and Haig Djambazian (Genome Quebec, McGill University, Montreal) for providing the myoblast cells and the DIC microscopy imaging of the cells. M. P. acknowledges financial support of the Spanish Ministry of Science postdoctoral fellows.

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Copyright information

© Springer Science+Business Media, LLC 2011

Authors and Affiliations

  • Mateu Pla-Roca
    • 1
  • David Juncker
    • 1
  1. 1.Bio-Medical Engineering DepartmentMcGill UniversityMontrealCanada

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