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Detection of GFP During Nervous System Development in Drosophila melanogaster

  • Karin Edoff
  • James S. Dods
  • Andrea H. Brand
Part of the Methods in Molecular Biology book series (MIMB, volume 411)

Abstract

Using the vital marker GFP and its spectral variants, it is possible to visualize multiple proteins in individual cells and thereby monitor embryonic development on a cellular and molecular level. In the following chapter we describe how to prepare Drosophila embryos or larvae for live imaging or immunohistochemical staining and provide some guidelines for optimal GFP detection.

Key Words

Green fluorescent protein fluorescent proteins nervous system development confocal microscopy 

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Copyright information

© Humana Press Inc., Totowa, NJ 2007

Authors and Affiliations

  • Karin Edoff
    • 1
    • 2
  • James S. Dods
    • 1
    • 2
  • Andrea H. Brand
    • 1
    • 2
  1. 1.Wellcome Trust/Cancer Research UK Gurdon InstituteUK
  2. 2.Department of Physiology, Development and NeuroscienceUniversity of CambridgeUK

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