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Methodologies for Staining and Visualisation of β-Galactosidase in Mouse Embryos and Tissues

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Part of the Methods in Molecular Biology book series (MIMB,volume 411)

Abstract

This chapter provides information on β-galactosidase staining of whole mouse embryos, organs, tissue sections, and cultured cells, as well as double staining with horseradish peroxidase and use as a tool for genotyping. Using these protocols, localization of β-galactosidase can be visualized throughout development and in adult tissues. β-Galactosidase staining may be used purely as a marker of gene expression and also as a tracer in cell lineage studies.

Key words

  • β-Galactosidase
  • embryo
  • tissues
  • cultured cells
  • immunohistochemistry

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  • DOI: 10.1007/978-1-59745-549-7_1
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© 2007 Humana Press Inc., Totowa, NJ

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Loughna, S., Henderson, D. (2007). Methodologies for Staining and Visualisation of β-Galactosidase in Mouse Embryos and Tissues. In: Anson, D.S. (eds) Reporter Genes. Methods in Molecular Biology, vol 411. Humana Press. https://doi.org/10.1007/978-1-59745-549-7_1

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  • DOI: https://doi.org/10.1007/978-1-59745-549-7_1

  • Publisher Name: Humana Press

  • Print ISBN: 978-1-58829-739-6

  • Online ISBN: 978-1-59745-549-7

  • eBook Packages: Springer Protocols