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Reporter Genes pp 1–11Cite as

Methodologies for Staining and Visualisation of β-Galactosidase in Mouse Embryos and Tissues

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Part of the Methods in Molecular Biology book series (MIMB,volume 411)

Abstract

This chapter provides information on β-galactosidase staining of whole mouse embryos, organs, tissue sections, and cultured cells, as well as double staining with horseradish peroxidase and use as a tool for genotyping. Using these protocols, localization of β-galactosidase can be visualized throughout development and in adult tissues. β-Galactosidase staining may be used purely as a marker of gene expression and also as a tracer in cell lineage studies.

Key words

  • β-Galactosidase
  • embryo
  • tissues
  • cultured cells
  • immunohistochemistry

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  • DOI: 10.1007/978-1-59745-549-7_1
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Author information

Authors and Affiliations

  1. School of Biomedical Sciences, University of Nottingham, UK

    Siobhan Loughna

  2. Institute of Human Genetics, University of Newcastle Upon Tyne, UK

    Dr Deborah Henderson

Authors
  1. Siobhan Loughna
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  2. Dr Deborah Henderson
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Editor information

Editors and Affiliations

  1. Department of Genetic Medicine, Women’s and Children’s Hospital, Adelaide, Australia

    Donald S. Anson

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© 2007 Humana Press Inc., Totowa, NJ

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Loughna, S., Henderson, D. (2007). Methodologies for Staining and Visualisation of β-Galactosidase in Mouse Embryos and Tissues. In: Anson, D.S. (eds) Reporter Genes. Methods in Molecular Biology, vol 411. Humana Press. https://doi.org/10.1007/978-1-59745-549-7_1

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  • DOI: https://doi.org/10.1007/978-1-59745-549-7_1

  • Publisher Name: Humana Press

  • Print ISBN: 978-1-58829-739-6

  • Online ISBN: 978-1-59745-549-7

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