Abstract
This chapter provides information on β-galactosidase staining of whole mouse embryos, organs, tissue sections, and cultured cells, as well as double staining with horseradish peroxidase and use as a tool for genotyping. Using these protocols, localization of β-galactosidase can be visualized throughout development and in adult tissues. β-Galactosidase staining may be used purely as a marker of gene expression and also as a tracer in cell lineage studies.
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© 2007 Humana Press Inc., Totowa, NJ
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Loughna, S., Henderson, D. (2007). Methodologies for Staining and Visualisation of β-Galactosidase in Mouse Embryos and Tissues. In: Anson, D.S. (eds) Reporter Genes. Methods in Molecular Biology, vol 411. Humana Press. https://doi.org/10.1007/978-1-59745-549-7_1
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DOI: https://doi.org/10.1007/978-1-59745-549-7_1
Publisher Name: Humana Press
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